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PURECUBE NI-NTA AGAROSE NI-NTA
首页 代理品牌 Cube Biotech HIS NI-NTA
PureCube Ni-NTA Agarose
产品简介
产品优势
产品详情
产品参数
相关参数
应用领域
产品简介
PureCube Ni-NTA Agarose

PureCube His Affinity Agarose (NTA)是针对组氨酸标签的亲和层析产品,组氨酸标签是应用广泛的亲和层析标签,这种标签具有小尺寸、低免疫原性的特点和在多种天然或变性条件下的广泛适用性,NTA相对于IDA具有更高的洗脱液纯度。

产品优势

(1)、德国制造,品质与信誉的坚实保障;

(2)、结合载量可高达80mg/mL,目前市面上非常高的结合能力;
(3)、更优越的DTT和EDTA稳定性和耐受性;
(4)、凝胶的多孔性和均匀粒径,使蛋白与凝胶之间有很好地相互作用;
(5)、适合低压环境下的纯化过程(大规模纯化制备或预装柱均可使用);
(6)、为研发、生产降低成本;
(7)、适用于大肠杆菌和真核细胞的裂解液和上清液;
(8)、同时可装载Co, Cu, Zn和其它金属离子。
产品详情

1. 收率高,纯度高


Fig. 1: Over 20% more yield obtained with PureCube Ni-NTA Agarose. SDS-PAGE of GFP expressed in E. coli and purified in gravity columns with PureCube Ni-NTA Agarose and Ni-NTA resin from Competitor Q. 80 mg/mL protein yield was obtained with PureCube Ni-NTA Agarose (E1–E4, Cube) compared to 65 and 48 mg/mL, respectively, with the widely used alternative resins G and Q (E1–E4, Competitor G / Competitor Q).


 

Cube Biotech

蛋白结合能力

≤80mg/mL



2. 优越的DTT和EDTA稳定性


Fig. 2: NTA is robust in the presence of reducing and chelating agents. GFP-His was purified on gravity columns containing PureCube Ni-NTA Agarose after exposing the resin for 1 h to 3 concentrations of DTT or EDTA. NTA exhibits a shallow decay rate in binding capacity.


PureCube Ni-NTA琼脂糖在DTT和EDTA存在下非常坚实,在稳定性测试中,将PureCube Ni-NTA琼脂糖置于DTT与EDTA浓度逐渐升高的环境中处理1小时。再用处理过的凝胶在重力层析柱中纯化大肠杆菌表达的GFP-His,凝胶的蛋白结合能力因存在DTT与EDTA而下降,但是衰减率平缓,即使在1.5 mM EDTA,凝胶依然具有其结合能力的54%。


 

Cube Biotech

DTT稳定性

<10mM

EDTA稳定性

<1.5mM

产品参数

应用

Specific binding and purification of 6x his-tagged proteins

特异性

Affinity to His-tagged proteins

结合能力

>80 mg/mL

Bead Ligand

Ni-NTA

粒径大小

40 μm

蝥合剂稳定性

Stable in buffer containing 10 mM DTT and 1 mM EDTA

保存

Delivered as a 50 % suspension

所需试剂耗材

Lysis Buffer Wash Buffer 

Elution Buffer 

Ice bath 

Refrigerated centrifuge for 50 mL tube (min 10,000 x g) 

50 mL centrifuge tube 

Micropipettor and Micropipetting tips

Disposable gravity flow columns with capped bottom outlet, 2 ml

pH meter

End-over-end shaker


SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment


相关参数

货号

产品

粒径

μm)

结合

载量

运输

温度

储存

温度

应用

31101

PureCube Ni-NTA Agarose(1ml)

40

80mg/mL

室温

4° C

适用于 His 标签重组蛋白的纯化 , 超高蛋白结合载量。pH 稳定工作范围2-14, 最大流速为6mL/min。 在10mM DTT 和1mM EDTA的环境下保持稳定

 

31103

PureCube Ni-NTA Agarose(10ml)

40

80mg/mL

室温

4° C

31105

PureCube Ni-NTA Agarose(50ml)

40

80mg/mL

室温

4° C

31110

PureCube Ni-NTA Agarose(250ml)

40

80mg/mL

室温

4° C

31112

PureCube Ni-NTA Agarose(500ml)

40

80mg/mL

室温

4° C

31115

PureCube Ni-NTA Agarose(1000ml)

40

80mg/mL

室温

4° C

31120

PureCube Ni-NTA Agarose(5000ml)

40

80mg/mL

室温

4° C



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