PureCube Ni-NTA Agarose
PureCube His Affinity Agarose (NTA)是针对组氨酸标签的亲和层析产品,组氨酸标签是应用广泛的亲和层析标签,这种标签具有小尺寸、低免疫原性的特点和在多种天然或变性条件下的广泛适用性,NTA相对于IDA具有更高的洗脱液纯度。
(1)、德国制造,品质与信誉的坚实保障;
(2)、结合载量可高达80mg/mL,目前市面上非常高的结合能力;(3)、更优越的DTT和EDTA稳定性和耐受性;
(4)、凝胶的多孔性和均匀粒径,使蛋白与凝胶之间有很好地相互作用;
(5)、适合低压环境下的纯化过程(大规模纯化制备或预装柱均可使用);
(6)、为研发、生产降低成本;
(7)、适用于大肠杆菌和真核细胞的裂解液和上清液;
(8)、同时可装载Co, Cu, Zn和其它金属离子。
1. 收率高,纯度高

| Cube Biotech |
蛋白结合能力 | ≤80mg/mL |
2. 优越的DTT和EDTA稳定性

Fig. 2: NTA is robust in the presence of reducing and chelating agents. GFP-His was purified on gravity columns containing PureCube Ni-NTA Agarose after exposing the resin for 1 h to 3 concentrations of DTT or EDTA. NTA exhibits a shallow decay rate in binding capacity.
PureCube Ni-NTA琼脂糖在DTT和EDTA存在下非常坚实,在稳定性测试中,将PureCube Ni-NTA琼脂糖置于DTT与EDTA浓度逐渐升高的环境中处理1小时。再用处理过的凝胶在重力层析柱中纯化大肠杆菌表达的GFP-His,凝胶的蛋白结合能力因存在DTT与EDTA而下降,但是衰减率平缓,即使在1.5 mM EDTA,凝胶依然具有其结合能力的54%。
| Cube Biotech |
DTT稳定性 | <10mM |
EDTA稳定性 | <1.5mM |
应用 | Specific binding and purification of 6x his-tagged proteins |
---|---|
特异性 | Affinity to His-tagged proteins |
结合能力 | >80 mg/mL |
Bead Ligand | Ni-NTA |
粒径大小 | 40 μm |
蝥合剂稳定性 | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
保存 | Delivered as a 50 % suspension |
所需试剂耗材 | Lysis Buffer Wash Buffer Elution Buffer Ice bath Refrigerated centrifuge for 50 mL tube (min 10,000 x g) 50 mL centrifuge tube Micropipettor and Micropipetting tips Disposable gravity flow columns with capped bottom outlet, 2 ml pH meter End-over-end shaker SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment |
货号 | 产品 | 粒径 (μm) | 结合 载量 | 运输 温度 | 储存 温度 | 应用 |
31101 | PureCube Ni-NTA Agarose(1ml) | 40 | 80mg/mL | 室温 | 4° C | 适用于 His 标签重组蛋白的纯化 , 超高蛋白结合载量。pH 稳定工作范围2-14, 最大流速为6mL/min。 在10mM DTT 和1mM EDTA的环境下保持稳定
|
31103 | PureCube Ni-NTA Agarose(10ml) | 40 | 80mg/mL | 室温 | 4° C | |
31105 | PureCube Ni-NTA Agarose(50ml) | 40 | 80mg/mL | 室温 | 4° C | |
31110 | PureCube Ni-NTA Agarose(250ml) | 40 | 80mg/mL | 室温 | 4° C | |
31112 | PureCube Ni-NTA Agarose(500ml) | 40 | 80mg/mL | 室温 | 4° C | |
31115 | PureCube Ni-NTA Agarose(1000ml) | 40 | 80mg/mL | 室温 | 4° C | |
31120 | PureCube Ni-NTA Agarose(5000ml) | 40 | 80mg/mL | 室温 | 4° C |
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Ewert, Jacob, et al.
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Buß, Maren et al.(2019). Specific high affinity interaction of Helicobacter pylori CagL with integrin α V β 6 promotes type IV secretion of CagA into human cells. The FEBS Journal. 10.1111/febs.14962.
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Furlan A.G. et al.
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Mayerthaler F. et al.
RSC Chem. Biol., 2021, Advance Article doi.org/10.1039/D0CB00220H
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