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PureCube Ni-NTA Agarose
PureCube His Affinity Agarose (NTA)是针对组氨酸标签的亲和层析产品，组氨酸标签是应用广泛的亲和层析标签，这种标签具有小尺寸、低免疫原性的特点和在多种天然或变性条件下的广泛适用性，NTA相对于IDA具有更高的洗脱液纯度。
(1)、德国制造，品质与信誉的坚实保障；
(2)、结合载量可高达80mg/mL，目前市面上非常高的结合能力；(3)、更优越的DTT和EDTA稳定性和耐受性；
(4)、凝胶的多孔性和均匀粒径，使蛋白与凝胶之间有很好地相互作用；
(5)、适合低压环境下的纯化过程（大规模纯化制备或预装柱均可使用）；
(6)、为研发、生产降低成本；
(7)、适用于大肠杆菌和真核细胞的裂解液和上清液；
(8)、同时可装载Co, Cu, Zn和其它金属离子。
1. 收率高，纯度高
| Cube Biotech |
蛋白结合能力 | ≤80mg/mL |
2. 优越的DTT和EDTA稳定性
Fig. 2: NTA is robust in the presence of reducing and chelating agents. GFP-His was purified on gravity columns containing PureCube Ni-NTA Agarose after exposing the resin for 1 h to 3 concentrations of DTT or EDTA. NTA exhibits a shallow decay rate in binding capacity.
PureCube Ni-NTA琼脂糖在DTT和EDTA存在下非常坚实，在稳定性测试中，将PureCube Ni-NTA琼脂糖置于DTT与EDTA浓度逐渐升高的环境中处理1小时。再用处理过的凝胶在重力层析柱中纯化大肠杆菌表达的GFP-His，凝胶的蛋白结合能力因存在DTT与EDTA而下降，但是衰减率平缓，即使在1.5 mM EDTA，凝胶依然具有其结合能力的54%。
| Cube Biotech |
DTT稳定性 | <10mM |
EDTA稳定性 | <1.5mM |
应用 | Specific binding and purification of 6x his-tagged proteins |
|---|---|
特异性 | Affinity to His-tagged proteins |
结合能力 | >80 mg/mL |
Bead Ligand | Ni-NTA |
粒径大小 | 40 μm |
蝥合剂稳定性 | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
保存 | Delivered as a 50 % suspension |
所需试剂耗材 | Lysis Buffer Wash Buffer Elution Buffer Ice bath Refrigerated centrifuge for 50 mL tube (min 10,000 x g) 50 mL centrifuge tube Micropipettor and Micropipetting tips Disposable gravity flow columns with capped bottom outlet, 2 ml pH meter End-over-end shaker SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment |
货号 | 产品 | 粒径 （μm） | 结合 载量 | 运输 温度 | 储存 温度 | 应用 |
31101 | PureCube Ni-NTA Agarose(1ml) | 40 | 80mg/mL | 室温 | 4° C | 适用于 His 标签重组蛋白的纯化 , 超高蛋白结合载量。pH 稳定工作范围2-14, 最大流速为6mL/min。 在10mM DTT 和1mM EDTA的环境下保持稳定
|
31103 | PureCube Ni-NTA Agarose(10ml) | 40 | 80mg/mL | 室温 | 4° C | |
31105 | PureCube Ni-NTA Agarose(50ml) | 40 | 80mg/mL | 室温 | 4° C | |
31110 | PureCube Ni-NTA Agarose(250ml) | 40 | 80mg/mL | 室温 | 4° C | |
31112 | PureCube Ni-NTA Agarose(500ml) | 40 | 80mg/mL | 室温 | 4° C | |
31115 | PureCube Ni-NTA Agarose(1000ml) | 40 | 80mg/mL | 室温 | 4° C | |
31120 | PureCube Ni-NTA Agarose(5000ml) | 40 | 80mg/mL | 室温 | 4° C |
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